Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | the enzyme requires reducing conditions for optimal activity. The omission of dithiothreitol from the enzymatic reactions reduces its activity by 70% | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fluoride | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.61 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal activity with 5 mM Mg2+. No activity with Mn2+ | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | Arabidopsis thaliana | - |
adenosine 5'-tetraphosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q52K88 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP | (2) | Arabidopsis thaliana | |
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-tetraphosphate + ADP | (1) | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | diadenosine tetraphosphate and diadenosine triphosphate, adenosine nucleotides, diphosphoinositol polyphosphate and phosphoribosyl pyrophosphate are not hydrolyzed | Arabidopsis thaliana | ? | - |
? | |
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O | P1,P5-bis(5'-adenosyl)pentaphosphate is hydrolyzed at 40% of the activity compared to P1,P6-bis(5'-adenosyl)hexaphosphate | Arabidopsis thaliana | adenosine 5'-tetraphosphate + AMP | - |
? | |
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | - |
Arabidopsis thaliana | adenosine 5'-tetraphosphate + ADP | - |
? | |
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | preferred substrate. Hydrolysis of P1,P6-bis(5'-adenosyl)hexaphosphate with the formation of ADP or AMP | Arabidopsis thaliana | adenosine 5'-tetraphosphate + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 26000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
At3g26690 | - |
Arabidopsis thaliana |
AtNUDT13 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.8 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Arabidopsis thaliana |
8.5 | - |
- |
Arabidopsis thaliana |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.07 | - |
pH 7.6, 37°C | Arabidopsis thaliana | fluoride |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C | Arabidopsis thaliana |